Program for GCSB 2019

2019-09-12
08:00
GCSB

Conference Registration

presented by GCSB

Registration opens at 8 am but also throughout the day, the registration desk will be occupied.

08:00 - 10:00 conference hall General Program
10:00
GCSB

Welcome by GASB e.V. and Dechema e.V.
Fabian Rohden and Wolfgang Wiechert

presented by GCSB

Fabian Rohden and Wolfgang Wiechert

10:00 - 10:15 conference hall General Program
10:15
GCSB

Aachen Verfahrenstechnik

presented by GCSB

Welcome Talk

10:15 - 10:30 conference hall General Program
10:30
GCSB

Ulrich Schwaneberg - Protein Engineering Keynote

presented by GCSB

KnowVolution: Redesigning Enzymes for Innovations Directed evolution has matured in academia and industry to a routinely applied algorithm to tailor protein properties to match application demands. In 2018, directed evolution methodologies were awarded with the Nobel Prize in chemistry due to their impact in biocatalysis and medicine1. In order to free directed enzyme evolution from method...

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10:30 - 11:00 conference hall Protein Engineering Session
11:00
GCSB

Julia Otten

presented by GCSB

Genetically encoded FRET-based biosensors for metabolite detection Metabolite detection in bacterial strain development is an important and demanding task. So far, most analysis is done via instrumental analysis or with enzymatic assays. However, these methods are often expensive, time consuming and can interfere with the process. An optical sensor utilizing fluorescence can be an easy opt...

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11:00 - 11:15 conference hall Protein Engineering Session
11:15
GCSB

Viktor Stein

presented by GCSB

iFLinkC: A Modular DNA Strategy for the Systematic Assembly and Recombination of Linker Peptides with Functional Domains Synthetic protein switches constitute critical components of artificial sensory systems that can sense, process and actuate molecular signals in a dynamic and autonomous fashion in real time. A key – yet, neglected aspect in the construction of synthetic protein switches...

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11:15 - 11:30 conference hall Protein Engineering Session
11:30
GCSB

Coffee Break

presented by GCSB

Coffee, Drinks, and food will be provided.

11:30 - 12:00 conference hall General Program
12:00
GCSB

Vitor Pinheiro - Xenobiology Keynote

presented by GCSB

Engineering new biological tools The same engineering principles underpinning Synthetic Biology also give rise to the paradigm of biology as a tool: capable of having its own specialisms and limitations. As a tool, driven by Darwinian evolution, Biology is clearly diverse but it has not and cannot explore all possibilities. It is feasible to extend, rewrite and even redesign biology with d...

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12:00 - 12:30 conference hall Xenobiology Session
12:30
GCSB

Tobias Pirzer

presented by GCSB

Towards synthetic cells using peptide-based reaction compartments Membrane compartmentalization and growth are central aspects of living cells, and are thus encoded in every cell's genome. For the creation of artificial cellular systems, genetic information and production of membrane building blocks will need to be coupled in a similar manner. However, natural biochemical reaction networks...

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12:30 - 12:45 conference hall Xenobiology Session
12:45
GCSB

Andreas Broedel

presented by GCSB

Protein engineering through directed evolution Although synthetic transcription factors enable the construction of complex artificial gene circuits, integrating multiple activating and repressing signals on single promoters remains a particular challenge. We highlight the first set of dual activator-repressor switches for orthogonal logic gates, based on bacteriophage λ cI variants and mul...

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12:45 - 13:00 conference hall Xenobiology Session
13:00
GCSB

SynBio Markets - Alex Williamson

presented by GCSB

Driving adoption of synthetic biology from lab to market across key verticals In recent years global investment in synthetic biology tools and applications has grown substantially. From $1.7 billion in 2017 to over $4 billion in 2018, and year to date investments of over $2 billion putting 2019 on course to match or exceed the previous year. Big US venture capital is leading the way, but t...

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13:00 - 13:15 conference hall General Program
13:15
GCSB

Lunch Break

presented by GCSB

Food and Drinks will be provided and are included in the ticket price.

13:15 - 14:30 conference hall General Program
14:30
GCSB

Panel Discussion Synthetic Biology in Industry

presented by GCSB

Panel Discussion with Dr. Andreas Meyer, Dr. Phillip Kuhn, Dr. Thorsten Sehl, and Alessandro Rossoni and short introductory talks by Dr. Andreas Meyer and Dr. Phillip Kuhn

Dr. Andreas Meyer Boosting the SynBio cycle in cell factory design with the nanoliter-reactor screening platform The application of synthetic biology in cell factory design relies on the well established DBTL (design...

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14:30 - 15:30 conference hall SynBio in Industry
15:30
GCSB

Coffee Break

presented by GCSB

Coffee and Drinks will be provided.

15:30 - 16:00 conference hall General Program
16:00
GCSB

Tobias Erb - Metabolic Engineering Keynote

presented by GCSB

Building an artificial chloroplast drop-by-drop.

One of the most fundamental transformations in Nature is the conversion of inorganic carbon (CO2) into living organic matter. This process literally feeds all life on earth. Although microorganism have evolved several enzymes and pathways to capture and convert CO2, the possible metabolic solution space of CO2-fixation has remained largel...

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16:00 - 16:30 conference hall Metabolic Engineering Session
16:30
GCSB

Christiane Katharina Sonntag

presented by GCSB

Modular Design Enables Fine-Tuning of Crucial Biosensor Parameters. Natural ligand-responsive transcription factors control the biosynthesis of small molecules, many of which are of biotechnological significance. As part of transcription factor-based biosensors, these regulators can be used to drive the expression of reporter genes, e.g. coding for fluorescent proteins, which are under con...

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16:30 - 16:45 conference hall Metabolic Engineering Session
16:45
GCSB

Simon Boecker

presented by GCSB

Broadening the Scope of Enforced ATP Wasting as a Tool for Metabolic Engineering ATP wasting has been proposed by us and other groups as a general tool to improve microbial cell factories in regard to their productivity and/or yield of a desired metabolite [1]. The approach consists of two steps: First, a knockout strategy has to be found in order to make the (by)production of a target com...

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16:45 - 17:00 conference hall Metabolic Engineering Session
17:00
GCSB

Coffee Break

presented by GCSB

Coffee and Drinks will be provided.

17:00 - 17:30 conference hall General Program
17:30
GCSB

Andreas Weber - Plant Synthetic Biology Keynote

presented by GCSB

Synthetic evolution of C4 photosynthesis C4 photosynthesis has independently and convergently evolved approx. 70 times in flowering plants. Most C4 plant species display a distinct leaf anatomy (Kranz-anatomy) and similar metabolic and biochemical features. Computational modeling indicates that C4 photosynthesis evolves from C3 photosynthesis via C3-C4 intermediate states [1,2]. C3-C4 inte...

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17:30 - 18:00 conference hall Plant Synthetic Biology Session
18:00
GCSB

Lars Milke

presented by GCSB

Tailoring Corynebacterium glutamicum towards increased malonyl-CoA availability for plant polyketide synthesis In the last years, different biotechnologically relevant microorganisms have been engineered for the synthesis of plant polyphenols such as flavonoids and stilbenes. However, low intracellular availability of malonyl-CoA as essential precursor for most plant polyphenols of inter...

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18:00 - 18:15 conference hall Plant Synthetic Biology Session
18:15
GCSB

Beau Brandon Dronsella

presented by GCSB

REDUCTIVE ACTIVITY OF 6-PHOSPHOGLUCONATE DEHYDROGENASE OFFERS A NEW CARBON FIXATION ROUTE The Calvin Cycle, the primary carbon fixation pathway in the biosphere, suffers from multiple drawbacks, including the low rate and lack of specificity of Rubisco. Multiple efforts were made to establish alternative carbon fixation reactions, some of which use novel reactions while others rely on exis...

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18:15 - 18:30 conference hall Plant Synthetic Biology Session
18:30
GCSB

Twist Bioscience

presented by GCSB

Synthetic Biology

18:30 - 18:45 conference hall General Program
18:45
GCSB

Dinner

presented by GCSB

Food and Drinks will be provided.

18:45 - 19:45 conference hall General Program
19:45
GCSB

Poster Session

presented by GCSB

Open Bar!

19:45 - 22:15 conference hall Poster Session
2019-09-13
08:45
GCSB

Welcome Coffee

presented by GCSB

We will provide coffee and tea!

08:45 - 09:15 conference hall General Program
09:15
GCSB

Jörn Kalinowski - Tools and Genome Engineering Keynote

presented by GCSB

Nanopore Sequencing – A Promising Application for Synthetic Biology in the Life Sciences Starting with a basic idea thirty years ago, nanopores have nowadays been engineered to perform sequencing in devices, you might use almost everywhere1. In contrast to the actual dominant sequencing technology, Sequencing-By-Synthesis (Illumina Inc.), nanopores offer single-molecule analysis and delive...

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09:15 - 09:45 conference hall Tools and Genome Engineering Session
09:45
GCSB

Katrin Messerschmidt

presented by GCSB

Artificial transcription factors and synthetic promoters for orthogonal protein expression in Saccharomyces cerevisiae Today, many products of our daily use are based on petroleum as mineral oil is a source of raw materials and energy. Besides diminishing sources of petroleum and mineral oil, using fossil resources generates pollution of the environment and climate changes. Certainly, mi...

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09:45 - 10:00 conference hall Tools and Genome Engineering Session
10:00
GCSB

Georg Hubmann

presented by GCSB

Measuring glycolytic flux in single yeast cells with an orthogonal synthetic biosensor Metabolic heterogeneity between individual cells of a population harbors extreme challenge for fundamental and applied research. Identifying metabolic heterogeneity and investigating its emergence requires tools to zoom into metabolism of individual cells. While methods exist to measure metabolite levels...

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10:00 - 10:15 conference hall Tools and Genome Engineering Session
10:15
GCSB

Coffee Break

presented by GCSB

Coffee and Drinks will be provided.

10:15 - 10:45 conference hall General Program
10:45
GCSB

Cornelia Palivan - Keynote Minimal and Cell-free Synthetic Biology

presented by GCSB

MIMICS OF NATURAL ORGANELLES BASED ON BIO-SYNTHETIC NANOCOMPARTMENTS WITH IN VIVO FUNCTIONALITY C.G. Palivana aChemistry Department, University of Basel, Mattenstrasse 24a, BPR-1096, POBox-3350, Basel, CH-4002, SWITZERLAND (cornelia.palivan@unibas.ch) New concepts that combine active compounds with stable, safe carriers or membranes resulting in functional systems are on focus in a v...

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10:45 - 11:15 conference hall Minimal and Cell-free Synthetic Biology Session
11:15
GCSB

Tom Robinson

presented by GCSB

High-throughput double emulsion synthesis of biomimetic cell-like compartments Giant unilamellar vesicles (GUVs) are used extensively in the field of biophysics to model cell membranes but more recently they have found a new use in bottom-up synthetic biology. Here the goal is to construct minimal cell systems from non-living components, and finding a suitable compartment to contain the re...

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11:15 - 11:30 conference hall Minimal and Cell-free Synthetic Biology Session
11:30
GCSB

Katrin Rosenthal

presented by GCSB

Cell-free protein synthesis: Accelerating biocatalyst development Cell-free protein synthesis (CFPS) has a wide range of applications as for example the synthesis of toxic or difficult-to-express proteins. CFPS is especially advantageous for screening many different proteins and to study the influence of genetic modifications on enzyme activity, stability, substrate and product specificity...

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11:30 - 11:45 conference hall Minimal and Cell-free Synthetic Biology Session
11:45
GCSB

Dispendix

presented by GCSB

Synthetic Biology

11:45 - 12:00 conference hall General Program
12:00
GCSB

Lunch Break

presented by GCSB

Food and Drinks will be provided and are included in the ticket price.

12:00 - 13:15 conference hall General Program
13:15
GCSB

Karl-Heinz Maurer - Legal and Regulatory Requirements for Synthetic Biology

presented by GCSB

Legal and Regulatory Requirements for Synthetic Biology

The future of genome editing after the decision by the European Court of Justice in 2018 are still high on everybody’s mind. Researchers might also be aware of the upcoming steps with the Nagoya protocol concerning sequence information. But we should be aware that beyond those very obvious topics there are significantly more issues...

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13:15 - 14:15 conference hall SynBio in Industry
14:15
GCSB

Coffee Break

presented by GCSB

Coffee and Drinks will be provided.

14:15 - 14:45 conference hall General Program
14:45
GCSB

Mustafa Khammash - Keynote Systems Biology, Computational Biology, and Genetic Networks

presented by GCSB

A universal biomolecular integral feedback controller for robust perfect adaptation

Homeostasis is a recurring theme in biology that ensures that regulated variables robustly—and in some systems, completely—adapt to environmental perturbations. This robust perfect adaptation feature is achieved in natural circuits by using integral feedback control. Despite its benefits, the synthetic re...

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14:45 - 15:15 conference hall Systems Biology, Computational Biology and Genetic Networks Session
15:15
GCSB

Zahra Razaghi-Moghadam

presented by GCSB

Feasibility of predicted metabolic engineering strategies Metabolic engineering relies on reaction manipulation (e.g. knock-out, over-expression, and down-regulation) to achieve as desired production of a target compound. Computational approaches have greatly contributed to design of metabolic engineering strategies on the level of reactions. However, these approaches neglect how the desir...

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15:15 - 15:30 conference hall Systems Biology, Computational Biology and Genetic Networks Session
15:30
GCSB

Ulf Liebal

presented by GCSB

Tool Development for Promoter Sequence Prediction with Defined Activity for sigma70-driven Gene Expression Development of synthetic pathways requires the fine-tuning of enzymatic activities for optimal operation. Commonly, strong promoters are used, potentially resulting in the accumulation of toxic intermediates, and induction of metabolic stress. The solution is to screen promoter sequen...

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15:30 - 15:45 conference hall Systems Biology, Computational Biology and Genetic Networks Session
15:45
GCSB

Coffee Break

presented by GCSB

Coffee and Drinks will be provided.

15:45 - 16:15 conference hall General Program
16:15
GCSB

Wilfried Weber - Keynote Mammalian and Medical Synthetic Biology

presented by GCSB

Extracellular Optogenetics to Control Mammalian Cell Fate and Function Optogenetics is impacting life science research and development in waves of innovation. With the introduction of light-sensitive ion channels into neuronal cells, the first wave of optogenetics enables fascinating insight and innovation in neurobiology. A similar wave occurred shortly after with the use of light-respons...

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16:15 - 16:45 conference hall Mammalian and Medical Synthetic Biology
16:45
GCSB

Priyanka Dhakane

presented by GCSB

Streptomyces albus based living therapeutic materials for sustained drug release Living therapeutics are microorganisms conceived for the treatment of diseases, most frequently through the production and release of a drug.[1] Synthetic biology tools allow genetic programming of bacteria to respond to external stimuli, such as temperature, pH, light or chemical inducers.[2] In this way, c...

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16:45 - 17:00 conference hall Mammalian and Medical Synthetic Biology
17:00
GCSB

Bertolt Gust

presented by GCSB

*Reconstruction of an entire biosynthetic gene cluster from Bacillus cereus VD169 to produce the antibiotic FR-900493 * The compound FR-900493 is a nucleoside antibiotic sharing basic structural elements with the well-studied caprazamycins and muraymycins. The mode of action is the inhibition of MraY translocase, an enzyme that catalyzes an essential intermediate step in peptidoglycan bio...

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17:00 - 17:15 conference hall Mammalian and Medical Synthetic Biology
17:15
GCSB

Closing Remarks

presented by GCSB

Summary of the GCSB and outlook to upcoming GASB and Dechema activities.

17:15 - 17:30 conference hall General Program
17:30
GCSB

GASB Members Meeting

presented by GCSB

Overview about GASB activities in the past and the future and election of the new steering committee.

17:30 - 18:30 conference hall GASB Members Meeting